Advanced
Mass Spectrometry
CHM
5938 (3) [Core Analytical Course]
Department
of Chemistry
Florida
International University
Instructor: Dr. Piero R. Gardinali� ������������������ Office:
������������������ CP-313� (305) 348-6354
������������������ ������������������ ��������� ��������� Lab:�� ��������� CP-348� (305)348-6249�
������������������ ������������������ ��������� ��������� e-mail:��������� gardinal@fiu.edu
Class Meets: �Tuesday and Thursday 17:00 - 18:15� CP-101
Course Description: The class offers a in
depth description of the processes and techniques involved in creating,
controlling and measuring elemental or molecular ionic species by mass
spectrometry techniques. Topics covered in the course include:� Theory of mass spectrometry. Methods of ionization.
Instrument Design. Combined Chromatography and Mass Spectrometry. Quantitative
aspects of mass spectrometry. Meta-stable ions in mass spectrometry. Recent
applications of mass spectrometry techniques (may involve some hands in
experiments).
Tentative Class outline:
Introduction to Mass
Spectrometry (2 classes)
� The mass spectrum
� Formation of ions
� Multipe Charged Species
� Isotopes
� Metastable Ions
� Elemental composition of Ions
� Apperance of the mass spectrum
Theory of Mass
Spectrometry (3 classes)
� Energy States and Ionization
� Formation of Ions
� Theories of fragmentation rates
� Ions lifetimes
� Qualitative Theories
Methods of ionization
(3-4 classes)
� Desorption Chemical Ionization (DCI)
� Negative-ion chemical ionization
� Field Desorption and Ionization
� Secondary Ion Mass Spectrometry (SIMS)
� Atmospheric Pressure Ionization
� Electrospray Ionization (ESI)
� Atmospheric Pressure Chemical Ionization (APCI)
� Matrix-Assisted Laser Desorption Ionization (MALDI)
Instrumental Design
(2-3 classes)
� Inlet systems
� Ion sources
� Mass analyzers (ion separators)
� Detection and recording of spectra
� Complete systems
Combined
chromatography and mass spectrometry (3 classes)
� Gas Chromatography/mass spectrometry (GC/MS)
� Liquid chromatography/mass spectrometry (LC/MS)
� Capillary electrophoresis/mass spectrometry (CE/MS)
� Supercritical fluid chromatography/mass spectrometry (SFC/MS)
� Thin-layer chromatography/mass spectrometry (TLC/MS)
� Other applications of mass spectrometry
Quantitative Mass
Spectrometry (2 classes)
� Introduction and principles
� Specificity
� Sensitivity and limits of detection
� Calibration and standards
� Selected ion monitoring (SIM)
� Selected reaction monitoring (SRM)
� Isotope dilution
� High-resolution vs. low-resolution
� Accurate mass measurements
� Derivatization
Metastable ions and
tandem mass spectrometry (2-3 classes)
� Unimolecular ion dissociation: Metastable ions
� Collision-induced Dissociation (CID)
� Double focusing magnetic sector instruments
� Triple Quadrupole instruments
� Three- and Four-sector instruments
� Hybrid instruments
� Time of flight instruments
� Ion traps
� Fourier transform instruments
� Tandem MS (MSn)
� Applications of MSn
Facilities tours,
Demonstrations, Student presentations, Assigned experiments-research
Required text (s): None
Suggested reading
material:
Books
Edmond De Hoffmann and Vincent
Stroobant , Mass Spectrometry : Principles and Applications ; 2nd Edition(2001). John Wiley
& Sons; New York, USA. ISBN: 0471485667
Johnstone, R.A.W., Mass Spectrometry for chemists and biochemists, 2nd Edition (1996) Cambridge University Press. USA. ISBN 0 521 42497 6
Journals
Analytical Chemistry
Organic Mass Spectrometry
International Journal of Mass Spectrometry and Ion Processes
Biological Mass Spectrometry
Mass Spectrometry Reviews
Journal of the American Society for Mass Spectrometry
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Grading:
Midterm Exam ����� ��������������� ��������������� 30%��������������� September 30th 2003 17:00-18:15 CP-101
Final Exam������������ ��������������� ��������������� 30%��������������� December 11th 15:30 � 18:15 CP-101
Paper/Presentation*���������� ��������������� 15%��������������� TBA
Assignments������� ��������������� ��������������� 15%��������������� 3 Times a semester
Paper Review������� ��������������� ��������������� 10%��������������� 1 time during the semester
*Depending on the number of
students in the class a term paper/presentation will be required.
Presentation: A 10 minute presentation on a �NOVEL� mass spectrometry topic is required.� Alternatively, a term paper (10 pages double space) on a� �NOVEL� mass spectrometry topic could be selected. All presentations must be made in Microsoft Power Point or equivalent software (I don't want to endorse anything!) no slides/overheads will be allowed! All final presentations will be compiled and distributed among the students at the end of the class in CD-ROM format.
Assignments are due a week from the day they are provided, at the beginning of the class period. No late submissions!
Once a semester, for the first 5 minutes of class, you will be asked to present a review of a research article that I will assign. You are required to provide a summary and a constructive critique on the topic to your classmates to start discussion on the topic.
IMPORTANT DATES:
NO-CLASSES������ ��������������� November 11 (Veterans Day); November 13 (SETAC); November 27 (Thanksgiving Holiday).
DROP�������������������� ��������������� October 18
LAST DAY OF CLASS��������������� December 4
TERM PAPER DUE ��������������� December 4