Advanced Mass Spectrometry

CHM 5938 (3) [Core Analytical Course]

Department of Chemistry

Florida International University

 

Instructor: Dr. Piero R. Gardinali������������������ Office: ������������������ CP-313(305) 348-6354

������������������ ������������������ ��������� ��������� Lab:�� ��������� CP-348(305)348-6249

������������������ ������������������ ��������� ��������� e-mail:��������� gardinal@fiu.edu

 

Class Meets: Tuesday and Thursday 17:00 - 18:15CP-101

 

Course Description: The class offers a in depth description of the processes and techniques involved in creating, controlling and measuring elemental or molecular ionic species by mass spectrometry techniques. Topics covered in the course include:Theory of mass spectrometry. Methods of ionization. Instrument Design. Combined Chromatography and Mass Spectrometry. Quantitative aspects of mass spectrometry. Meta-stable ions in mass spectrometry. Recent applications of mass spectrometry techniques (may involve some hands in experiments).

 

 

Tentative Class outline:

 

Introduction to Mass Spectrometry (2 classes)

 

   The mass spectrum

   Formation of ions

   Multipe Charged Species

   Isotopes

   Metastable Ions

   Elemental composition of Ions

   Apperance of the mass spectrum

 

Theory of Mass Spectrometry (3 classes)

 

   Energy States and Ionization

   Formation of Ions

   Theories of fragmentation rates

   Ions lifetimes

   Qualitative Theories

 

Methods of ionization (3-4 classes)

 

   Gas-Phase Ionization

   Electron Ionization (EI)

   Chemical Ionization (CI)

   Desorption Chemical Ionization (DCI)

   Negative-ion chemical ionization

   Field Desorption and Ionization

   Field Desorption (FD)

   Field Ionization (FI)

   Particle Bombardment

   Fast Atom Bombardment (FAB)

   Secondary Ion Mass Spectrometry (SIMS)

   Atmospheric Pressure Ionization

   Electrospray Ionization (ESI)

   Atmospheric Pressure Chemical Ionization (APCI)

   Laser Desorption

   Matrix-Assisted Laser Desorption Ionization (MALDI)

 

MIDTERM EXAM ��������������� September 30 2003 17:00-18:15 PM CP101

 

Instrumental Design (2-3 classes)

 

   Inlet systems

   Ion sources

   Mass analyzers (ion separators)

   Detection and recording of spectra

   Complete systems

 

Combined chromatography and mass spectrometry (3 classes)

 

   Gas Chromatography/mass spectrometry (GC/MS)

   Liquid chromatography/mass spectrometry (LC/MS)

   Capillary electrophoresis/mass spectrometry (CE/MS)

   Supercritical fluid chromatography/mass spectrometry (SFC/MS)

   Thin-layer chromatography/mass spectrometry (TLC/MS)

   Other applications of mass spectrometry

 

Quantitative Mass Spectrometry (2 classes)

 

   Introduction and principles

   Specificity

   Sensitivity and limits of detection

   Calibration and standards

   Selected ion monitoring (SIM)

   Selected reaction monitoring (SRM)

   Isotope dilution

   High-resolution vs. low-resolution

   Accurate mass measurements

   Derivatization

 

Metastable ions and tandem mass spectrometry (2-3 classes)

 

   Unimolecular ion dissociation: Metastable ions

   Collision-induced Dissociation (CID)

   Double focusing magnetic sector instruments

   Triple Quadrupole instruments

   Three- and Four-sector instruments

   Hybrid instruments

   Time of flight instruments

   Ion traps

   Fourier transform instruments

   Tandem MS (MSn)

   Applications of MSn

 

Facilities tours, Demonstrations, Student presentations, Assigned experiments-research

 

Required text (s): None

 

 

Suggested reading material:

 

Books

 


Edmond De Hoffmann and Vincent Stroobant , Mass Spectrometry : Principles and Applications ; 2nd Edition(2001). John Wiley & Sons; New York, USA. ISBN: 0471485667

QD96.M3 H6413 2001

 

Johnstone, R.A.W., Mass Spectrometry for chemists and biochemists, 2nd Edition (1996) Cambridge University Press. USA. ISBN 0 521 42497 6

QD96.M3 R67 1996

 

Journals

Analytical Chemistry

Organic Mass Spectrometry

International Journal of Mass Spectrometry and Ion Processes

Biological Mass Spectrometry

Mass Spectrometry Reviews

Rapid Communications in Mass Spectrometry

Journal of the American Society for Mass Spectrometry

 

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Grading:

 

Midterm Exam ����� ��������������� ��������������� 30%��������������� September 30th 2003 17:00-18:15 CP-101

Final Exam������������ ��������������� ��������������� 30%��������������� December 11th 15:30 � 18:15 CP-101

Paper/Presentation*���������� ��������������� 15%��������������� TBA

Assignments������� ��������������� ��������������� 15%��������������� 3 Times a semester

Paper Review������� ��������������� ��������������� 10%��������������� 1 time during the semester

 

*Depending on the number of students in the class a term paper/presentation will be required.

 

 

 

Presentation: A 10 minute presentation on a �NOVEL� mass spectrometry topic is required.Alternatively, a term paper (10 pages double space) on a�NOVEL� mass spectrometry topic could be selected. All presentations must be made in Microsoft Power Point or equivalent software (I don't want to endorse anything!) no slides/overheads will be allowed! All final presentations will be compiled and distributed among the students at the end of the class in CD-ROM format.

 

Assignments are due a week from the day they are provided, at the beginning of the class period. No late submissions!

 

Once a semester, for the first 5 minutes of class, you will be asked to present a review of a research article that I will assign. You are required to provide a summary and a constructive critique on the topic to your classmates to start discussion on the topic.

 

IMPORTANT DATES:

 

NO-CLASSES������ ��������������� November 11 (Veterans Day); November 13 (SETAC); November 27 (Thanksgiving Holiday).

 

DROP�������������������� ��������������� October 18

 

LAST DAY OF CLASS��������������� December 4

 

TERM PAPER DUE ��������������� December 4