• Publications

    2018

    (73) Canoura J., Wang Z.W., Yu H.X., Alkhamis O., Fu F.F. & Xiao Y. "No Structure-switching required: A generalizable exonuclease-mediated aptamer-based assay for small-molecule detection." J. Am. Chem. Soc. 140, 9961– 9971, 2018.

    (72) Wang Z.W., Yu H.X., Canoura J., Liu Y.Z., Alkhamis O., Fu, F.F. & Xiao Y. "Introducing structure-switching functionality into small-molecule-binding aptamers via nuclease-directed truncation.” Nucleic Acids Res. 46, e81, 2018.

    (71) Yu H.X., Yang W.J., Alkhamis O., Canoura J., Yang, K. & Xiao Y. "In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality." Nucleic Acids Res. 46, e43, 2018.

    (70) Liang P.P., Canoura J., Yu H.X., Alkhamis O. & Xiao Y. "Dithiothreitol-regulated coverage of oligonucleotide-modified gold nanoparticles to achieve optimized biosensor performance." ACS Applied Materials & Interfaces 10, 4233 – 4242, 2018.

    (69) Yu H.X., Canoura J., Guntupalli B., Alkhamis O. & Xiao Y. "Rapid and sensitive detection of small-molecule targets using cooperative binding split aptamers and enzyme-assisted target recycling." Anal. Chem. 90, 1748 – 1758, 2018.

    2017

    (68) Yu H.X., Xu X.W., Liang P.P., Loh K.Y., Guntupalli B., Roncancio D. & Xiao Y. "A broadly-applicable assay for rapidly and accurately quantifying DNA surface coverage on diverse particles." Bioconjugate Chem. 28, 933 – 943, 2017.

    (67) Yu, H.X., Canoura J., Guntupalli B., Lou X.H. & Xiao Y. "A Cooperative-Binding Split Aptamer Assay for Rapid, Specific and Ultra-Sensitive Fluorescence Detection of Cocaine in Saliva." Chem. Sci. 8, 131 – 141, 2017

    2016

    (66) Xu Q., Lou X.H., Wang L., Ding X.F., Yu H.X. & Xiao Y. "Rapid, surfactant-free, and quantitative functionalization of gold nanoparticles with thiolated DNA under physiological pH and its application in molecular beacon-based biosensor." ACS Appl. Mater. Interfaces. 8, 27298 – 27304, 2016.

    2015

    (65) Guntupalli B., Liang P.P., Lee, J.H., Yang Y.H., Yu, H.X., Canoura J., He J., Li W.Z., Weizmann Y. & Xiao Y. "Ambient Filtration Method to Rapidly Prepare Highly Conductive, Paper-Based Porous Gold Films for Electrochemical Biosensing." ACS Appl. Mater. Interfaces. 7, 27049-27058, 2015.

    (64) Zhao T., Liu R., Yu H.X., Zhao J.C., Wang X., Lou X.H. & Xiao Y.  “A Nanoprobe-Enhanced, Split Aptamer-Based Electrochemical Sandwich Assay for Ultrasensitive Detection of Small Molecules.” Anal. Chem. 87, 7712-7719, 2015.

    (63) Liang P.P., Yu H.X. Guntupalli B. & Xiao Y.  “Paper-Based Device for Rapid Visualization of NADH Based on Dissolution of Gold Nanoparticles.” ACS Appl. Mater. Interfaces. 7, 15023-15030, 2015.

    2014

    (62) Roncancio D., Yu H.X., Xu X.W., Wu S., Liu R., Debord J., Lou X.H. & Xiao Y.  “A Label-Free Aptamer-Fluorophore Assembly for Rapid and Specific Detection of Cocaine in Biofluids.” Anal. Chem. 86, 11100-11106 2014.

    (61) Wu S., Liang P.P., Yu H.X., Xu X.W., Liu Y., Lou X.H. & Xiao Y.  “Amplified Single Base-Pair Mismatch Detection via Aggregation of Exonuclease-Sheared Gold Nanoparticles.” Anal. Chem. 86, 3461-3467 2014.

    2013

    (60) Lou X.H., Zhao T., Liu R., Ma, J.S. & Xiao Y. “Self-Assembled DNA Monolayer Buffered Dynamic Ranges of Mercuric Electrochemical Sensor.” Anal. Chem. 85, 7574-7580, 2013.

    (59) Oh S.S., Plakos K., Xiao Y. & Soh H.T.  “In Vitro Selection of Shape-Changing DNA Nanostructures Capable of Binding-Induced Cargo Release.” ACS Nano. 7, 9675-9683, 2013.

    (58) Deborggraeve S., Dai J.Y., Xiao Y. & Soh H.T. “Controlling the Function of DNA Nanostructures with Specific Trigger Sequences.” Chem. Comm. 49, 397-399, 2013.

    2012

    (57) Ahmad K.M., Xiao Y. & Soh H.T.  “Selection is More Intelligent Than Design: Improving the Affinity of a Bivalent Aptamer Through Directed Evolution.” Nucleic Acids Res. 40, 11777-117843, 2012.

    (56) Feng L.Y., Zhao C.Q., Xiao Y., Wu L., Ren J.S. & Qu X.G. “Electrochemical DNA Three-Way Junction Based Sensor for Distinguishing Chiral Metallo-Supramolecular Complexes.” Chem. Comm. 48, 6900-6902, 2012.

    2011

    (55) Olmsted I.R., Xiao Y., Cho M., Csordas A., Sheehan J.H, Meiler J., Soh H.T. & Bornhop D.J.   “Measurement of Aptamer-Protein Interactions with Back-Scattering Interferometry.” Anal. Chem. 83, 8867-8870, 2011.

    (54) Ahmad K.M., Oh S.S., Kim S., Xiao Y. & Soh H.T. “Probing the Limits of Aptamer Affinity with a Microfluidic SELEX Platform.”  PLoS One 6, e27051, 2011.

    (53) Zuo X.L., Xia F., Patterson A., Soh H.T., Xiao Y. & Plaxco K.W. “Two-Step, PCR-Free Telomerase Detection by Using Exonuclease III-Aided Target Recycling.” ChemBioChem. 12, 2745-2747, 2011.

    (52) Hsieh K.W., White R.J., Ferguson B.S., Xiao Y. & Soh H.T. “Polarity-Switching Electrochemical Sensor for Specific Detection of Single-Nucleotide Mismatches.” Angew. Chem. Int. Ed. 50, 11176-11180, 2011.

    (51) Oh S.S., Ahmad K.M., Cho M., Kim S., Xiao Y. & Soh H.T. “Improving Aptamer Selection Efficiency Through Volume Dilution, Magnetic Concentration, and Continuous Washing in Microfluidic Channels.” Anal. Chem. 83, 6883-6889, 2011.

    (50) Ferguson B.S., Buchsbaum S.F., Wu T.-T., Hsieh K., Xiao Y., Sun R. & Soh H.T. “Integrated Genetic Analysis of H1N1 Influenza Virus in a Monolithic Microfluidic System.” J. Am. Chem. Soc. 133, 9129-9135, 2011.

    (49) Wang J.P., Liu Y.L., Teesalu T., Sugahara K., Kotamrajua V.R., Adams J.D., Ferguson B.S., Gong Q., Oh S.S., Csordas A.T., Ruoslahti E., Xiao Y. & Soh H.T. “Selection of Phage-Displayed Peptides on Live Adherent Cells in Microfluidic Channels.” Proc. Natl. Acad. Sci. U.S.A. 108, 6909–6914, 2011.

    2010

    (48) Xiao Y., Dane K.Y., Uzawa T., Csordas A., Qian J.R., Soh H.T., Daugherty P.S., Lagally E., Heeger A.J. & Plaxco K.W. “Detection of Telomerase Activity in High Concentration of Cell Lysates Using Primer-Modified Gold Nanoparticles.”  J. Am. Chem. Soc. 132, 15299-15307, 2010.  

    (47) Xia F., White R.J., Zuo X.L., Patterson A., Xiao Y., Kang D., Gong X., Plaxco K.W. & Heeger A.J.  “An Electrochemical Super-Sandwich Assay for Sensitive and Selective DNA Detection in Serum.” J. Am. Chem. Soc. 132, 14346-14348, 2010.

    (46) Cho M., Xiao Y., Nie J., Stewart R., Csordas A., Oh S.S., Thomson J.A. & Soh H.T. “Quantitative Selection of DNA Aptamers Through Microfluidic Selection and High Throughput Sequencing.”  Proc. Natl. Acad. Sci. U.S.A. 107, 15373-15378, 2010. 

    (45) Xia F., Zuo X.L., Yang R.Q., Ryan White R., Xiao Y., Kang D., Gong X., Lubin A.A., Vallée-Bélisle A., Yuen J.D., Hsu B.Y. & Plaxco K.W.  “Label-Free, Dual-Analyte Electrochemical Biosensors: A New Class of Molecular-Electronic Logic Gates.” J. Am. Chem. Soc. 132, 8557-8559, 2010. 

    (44) Xia F., Zuo X.L., Yang R.Q., Xiao Y., Kang D., Vallée-Bélisle A., Gong X., Yuen J.D., Hsu B.Y., Heeger A.J. & Plaxco K.W. “Colorimetric Detection of DNA, Small Molecules, Proteins and Ions, using Unmodified Gold Nanoparticles and Conjugated Polyelectrolytes.”  Proc. Natl. Acad. Sci. U.S.A. 107, 10837-10841, 2010. 

    (43) Oh S.S., Plakos K., Lou X.H., Xiao Y. & Soh H.T. “In Vitro Selection of Structure Switching, Self-Reporting Aptamers.” Proc. Natl. Acad. Sci. U.S.A. 107, 14053-14058, 2010.

    (42) Hsieh K.W., Xiao Y. & Soh H.T. “Electrochemical DNA Detection via Exonuclease and Target-Catalyzed Transformation of Surface-Bound Probes.” Langmuir 26, 10392-10396, 2010.

    (41) Zuo X.L., Xia F., Xiao Y. & Plaxco K.W.  “A Sensitive and Selective, Amplified Fluorescence DNA Detection Based on Exonuclease III Aided Target Recycling.” J. Am. Chem. Soc. 132, 1816-1818, 2010.

    (40) Xia F., Zuo X.L., Yang R.Q., Xiao Y. ,Kang D., Vallée-Bélisle A., Gong X., Heeger A.J. & Plaxco K.W.  “On the Binding of Cationic, Water-Soluble Conjugated Polymer to DNA: Electrostatic and Hydrophobic Interactions.”  J. Am. Chem. Soc. 132, 1252-1254, 2010.

    (39) Csordas A., Gerdon A.E., Adams J.D., Qian J.R., Oh S.S., Xiao Y. & Soh H.T.  “Detection of Proteins in Serum via Micromagnetic Aptamer PCR (MAP) Technology.” Angew. Chem. Int. Ed. 49, 355-358, 2010.

    2009

    (38) Xiao Y., Lou X.H., Uzawa T., Plakos K., Plaxco K.W. & Soh H.T.  “An Electrochemical Sensor for Single Nucleotide Polymorphism Detection in Serum Based on a Triple-Stem DNA probe.” J. Am. Chem. Soc. 131, 15311-15316, 2009.

    (37) Peng Y.H., Wang X.H., Xiao Y., Feng L.Y., Zhao C., Ren J.S. & Qu X.G. “i-Motif Quadruplex DNA-Based Biosensor for Distinguishing Single- and Multiwalled Carbon Nanotubes.” J. Am. Chem. Soc. 131, 13813-13818, 2009.

    (36) Lou X.H., Xiao Y. ,Wang Y., Mao H.G. & Zhao, J.L. “Label-Free Colorimetric Screening of Exonuclease and Endonuclease Activity Using Unmodified Gold Nanoparticles.” ChemBioChem. 10, 1973-1977, 2009.

    (35) Qian J.R., Lou X.H., Zhang Y.T., Xiao Y. & Soh H.T. “Generation of Highly Specific Aptamers via Micromagnetic Selection.” Anal. Chem. 81, 5490-5495, 2009.

    (34) Zuo X.L., Xiao Y. & Plaxco K.W. “High Specificity, Electrochemical Sandwich Assays Based on Single Aptamer Sequences and Suitable for the Direct Detection of Small-Molecule Targets in Blood and Other Complex Matrices.” J. Am. Chem. Soc. 131, 6944-6945, 2009.

    (33) Xiao Y., Plakos K., Lou X.H., White R.J., Plaxco K.W. & Soh H.T. “Fluorescence Detection of Single Nucleotide Polymorphism via a Single Self-Complementary, Triple-Stem DNA Probe.” Angew. Chem. Int. Ed. 48, 4354-4358, 2009.

    (32) Xiao Y., Uzawa T., White R.J., DeMartini D. & Plaxco K.W.  “On the Signaling of Electrochemical Aptamer-Based Sensors: Collision- and Folding-Based Mechanisms.”  Electroanalysis 21, 1267-1271, 2009

    (31) Swensen J.S., Xiao Y., Ferguson B.S., Lubin A.A., Lai R.Y., Heeger A.J., Plaxco K.W. & Soh H.T.  “Continuous, Real-Time Monitoring of Cocaine in Undiluted Blood Serum via a Microfluidic Electrochemical Aptamer-Based Sensor.”  J. Am. Chem. Soc. 131, 4262-4266, 2009.

    (30) Lou X.H., Qian J.R., Xiao Y., Viel L., Gerdon A.E., Lagally E.T., Atzberger P., Heeger A.J. & Soh H.T.  “Micromagnetic Selection of Aptamers in Microfluidic Channels.” Proc. Natl. Acad. Sci. U.S.A. 106, 2989-2994, 2009.

    (29) Xiao Y. & Plaxco K.W. “Electrochemical Aptamer Sensors.” In: Functional Nucleic Acids for Sensing and Other Analytical Applications. Y. Lu and Y. Li, eds. Kluwer/Springer.

    (28) Cash J.K., Heeger A.J., Plaxco K.W. & Xiao Y. “Optimization of a Reusable, DNA Pseudoknot-Based Electrochemical Sensor for Sequence-Specific DNA Detection in Blood Serum.” Anal. Chem. 81, 656-661, 2009.

    2008-2007

    (27) White R.J., Phares N., Lubin A.A., Xiao Y. & Plaxco, K.W.  “Optimization of Electrochemical Aptamer-Based Sensors via Optimization of Probe Packing Density and Surface Chemistry.”  Langmuir 24, 10513-10518, 2008.

    (26) Xiao Y., Lai R. & Plaxco K.W. “Preparation of Electrode-Immobilized, Redox-Modified Oligonucleotides for Electrochemical DNA and Aptamers-Based Sensing.” Nature Protocol 2, 2875-2880, 2007.

    (25) Xiao Y., Qu X.G., Plaxco K.W. & Heeger A.J. “Label-Free Electrochemical Detection of DNA in Blood Serum by Target-Induced Resolution of an Electrode-Bound Pseudoknot DNA.”  J. Am. Chem. Soc. 129, 11896-11896, 2007.

    (24) Xiao Y., Rowe A.A. & Plaxco K.W. “Electrochemical Detection of Parts Per Billion Lead via an Electrode-Bound DNAzyme Assembly.”  J. Am. Chem. Soc. 129, 262-263, 2007.

    2006-2005

    (23) Xiao Y., Lubin A.A., Baker B.R., Plaxco K.W. & Heeger A.J. “Single-Step Electronic Detection of Femtomolar DNA by Target-Induced Strand Displacement in an Electrode-Bound Duplex.”  Proc. Natl. Acad. Sci. U.S.A. 103, 16677-16680, 2006.

    (22) Xiao Y., Lubin A.A., Heeger A.J. & Plaxco K.W. “Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor.” Angew. Chem. Int. Ed. 44, 5456-5459, 2005.

    (21) Xiao Y., Piorek B.D., Plaxco K.W. & Heeger A.J.  “A Reagentless Signal-On Architecture for Electronic, Aptamer-Based Sensors via Target-Induced Strand Displacement.”  J. Am. Chem. Soc. 127, 17990-17991, 2005.

    (20) Xiao Y., Shlyahovsky B., Popov I., Pavlov V. & Willner I. “Shape and Color of Au Nanoparticles Follow Biocatalytic Processes.”  Langmuir 21, 5659-5662, 2005.

    (19) Xiao Y., Pavlov V., Shlyahovsky B. & Willner I. “An Os-II-Bisbipyridine-4-Picolinic Acid Complex Mediates the Biocatalytic Growth of Au Nanoparticles: Optical Detection of Glucose and Acetylcholine Esterase Inhibition.”  Chemistry-A European Journal 11, 2698-2704, 2005.

    (18) Pavlov V., Xiao Y. & Willner I. “Inhibition of the Acetycholine Esterase-Stimulated Growth of Au Nanoparticles: Nanotechnology-Based Sensing of Nerve Gases.” Nano Lett. 5, 649-653, 2005.

    (17) Shlyahovsky B., Katz E., Xiao Y., Pavlov V. & Willner I. “Optical and Electrochemical Detection of NADH and of NAD(+)-Dependent Biocatalyzed Processes by the Catalytic Deposition of Copper on Gold Nanoparticles.”  Small 1, 213-216, 2005.

    2004-2003

    (16) Xiao Y., Pavlov V., Levine S., Niazov T., Markovitch G. & Willner I. “Catalytic Growth of Au Nanoparticles by NAD(P)H Cofactors: Optical Sensors for NADP(+)-Dependent Biocatalyzed Transformations.”  Angew. Chem. Int. Ed. 43, 4519-4522, 2004.

    (15) Xiao Y., Pavlov V., Niazov T., Dishon A., Kotler M. & Willner I. “Catalytic Beacons for the Detection of DNA and Telomerase Activity.”  J. Am. Chem. Soc. 126, 7430-7431, 2004.

    (14) Pavlov V., Xiao Y., Shlyahovsky B. & Willner I.  “Aptamer-Functionalized Au Nanoparticles for the Amplified Optical Detection of Thrombin.”  J. Am. Chem. Soc. 126, 11768-11769, 2004.

    (13) Pavlov V., Xiao Y., Gill R., Dishon A., Kotler M. & Willner I.  “Amplified Chemiluminescence Surface Detection of DNA and Telomerase Activity using Catalytic Nucleic Acid Labels.”  Anal. Chem. 76, 2152-2156, 2004.

    (12) Shi L.X., Xiao Y. & Willner I. “Electrical Contacting of Glucose Oxidase by DNA-Templated Polyaniline Wires on Surfaces.”  Electrochem. Commun. 6 , 1057-1060, 2004.

    (11) Niazov T., Pavlov V., Xiao Y., Gill R. & Willner I.  “DNAzyme-Functionalized Au Nanoparticles for the Amplified Detection of DNA or Telomerase Activity.”   Nano Lett. 4, 1683-1687, 2004.

    (10) Xiao Y., Pavlov V., Gill R. & Willner I.  “Lighting Up Biochemiluminescence by Surface-Assembly of DNA-Hemin Complex.”   ChemBioChem. 5, 374-379, 2004.

    (9) Xiao Y., Patolsky F., Katz E., Hainfeld J.F. & Willner I. “”Plugging into Enzymes”: Nanowiring of Redox Enzymes by a Gold Nanoparticles.”   Science 299, 1877-1881, 2003.

    (8) Xiao Y., Kharitonov A.B., Patolsky F., Weizmann Y. & Willner I. “Electrocatalytic Intercalator-Induced Winding of Double-Stranded DNA with Polyaniline.”  Chem. Commun. 13, 1540-1541, 2003.

    2002-1998

    (7) Ju H.X., Xiao Y., Lu X.J. & Chen H.Y. “Electrooxidative Coupling of a Toluidine Blue Terminated Self-Assembled Monolayer Studied by Electrochemistry and Surface Enhanced Raman Spectroscopy.”  J. Electroanal. Chem. 518, 123-130, 2002.

    (6) Hu X.Y, Xiao Y. & Chen H.Y. “Anion Adsorption on an Au Colloid Monolayer Based Cysteamine-Modified Gold Electrode.”   Chem. Res. Chin. Univ. 17, 159-167, 2001.

    (5) Xiao Y., Ju H.X. & Chen H.Y. “Direct Electrochemistry of Horseradish Peroxidase Immobilized on a Colloid/Cysteamine-Modified Gold Electrode.” Anal. Biochem. 278, 22-28, 2000.

    (4) Xiao Y., Ju H.X. & Chen H.Y. “Hydrogen Peroxide Sensor Based on Horseradish Peroxidase-Labeled Au Colloids Immobilized on Gold Rlectrode Surface by Cysteamine Monolayer.” Anal. Chim. Acta 391, 73-82, 1999.

    (3) Xiao Y., Ju H.X. & Chen H.Y. “A Reagentless Hydrogen Peroxide Sensor Based on Incorporation of Horseradish Peroxidase in Poly(thionine) Film on a Monolayer Modified Electrode.” Anal. Chim. Acta 391, 299-306, 1999.

    (2) Hu X.Y., Xiao Y. & Chen H.Y. “Adsorption Characteristics of Fe(CN)(3)(-/4)(6) on Au Colloids as Monolayer Films on Cysteamine-Modified Gold Electrode.” J. Electroanal. Chem. 466, 26-30, 1999.

    (1) Ju H.X., Zhou D.M., Xiao Y. & Chen H.Y. “Amperometric Biosensor for Glucose Based on a Nanometer-Sized Microband Gold Electrode Coimmobilized with Glucose Oxidase and Poly(o-Phenylenediamide).”  Electroanalysis 10, 541-545, 1998.

  • Patents

    (7) Liang P.P., Yu H.X. & Xiao Y. “Materials and methods for rapid visualization of NAD(P)H.”  Patent No. US 10000790

    (6) Xiao Y., Guntupalli B. & Paudyal J. “Highly conductive porous paper-based metal thin films”. Patent No. US 20160329121.

    (5) Roncancio D., Yu H.X., Xu X.W. & Xiao Y. “Materials and Methods for Rapid and Specific Detection of Cocaine”. Patent No. US 20160131668.

    (4) Soh H.T. & Xiao Y. “Target Detection Using a Single-Stranded Self-Complementary, Triple-Stem DNA Probe.”  Application number: US2009060543

    (3) Xiao Y., Lubin A.A. & Plaxco K.W. “Signal-on Architecture for Electronic, Oligonucleotide-Based Sensors.” Application number: US2007154909

    (2) Willner I., Pavlov V., Zayatz M., Niazov T., Shlyahovsky B., Xiao Y. ,& Buron R. “Novel Sensors with Nanoparticle Probe.” Application number: WO2006008742

    (1) Willner I., Xiao Y., Pavlov V., Gill R. & Niazov T.  “Catalytic Polynucleotide and its Use for Determination of Analytes.” Application number: US2007254282