Graduate Students wanted!

Posted by | Education, Epigenetics, FIU, Histones, Marine Biology, Omics, Research | No Comments

Graduate Student (PhD) positions available in Dr. Eirin-Lopez’s Chromatin Structure & Evolution Lab (Department of Biological Sciences, Florida International University) starting in Fall 2016.

We are looking for enthusiastic, dynamic and independent students broadly interested in studying the interface between evolution, epigenetics and adaptation in marine organisms. Students would ideally have a B.S. degree in Biology or related discipline (academic training in biology, statistics, genetics and evolution) and must be proficient in English (both spoken and written). Candidates with additional knowledge on computer science and/or bioinformatics are encouraged to apply. Our research addresses the study of chromatin and epigenetics from different perspectives, most notably evolution, development and adaptation. To this end we use marine invertebrates as model systems in the lab, as well as a wide range of eukaryote groups in molecular evolutionary analyses. Our current projects combine elements from marine biology, toxicology, molecular biology, biochemistry, next generation sequencing, bioinformatics and molecular evolution to address environmental problems in the oceans. Our work requires good organizational and computational skills and the ability to work collaboratively as part of a team. Detailed information about our research is available at our website (chromevol.com). Follow us in Facebook (facebook.com/chromevol) and Twitter (@chromevol)

Potential research topics include (but are not limited to):

1) Characterization of epigenetic mechanisms underlying environmental responses and adaptation in marine invertebrates. This research addresses the roles of DNA methylation, histone variant modifications and other epigenetic mechanisms during responses to environmental stressors, notably by using bivalve molluscs and corals as model organisms.

2) Development of high-throughput “-omic” tools improving pollution biomonitoring in coastal areas. This topic focuses on the development of methods that can be routinely used to identify early exposure of marine organisms to genotoxic pollutants (e.g., microarrays, qPCR, proteomic analyses, etc.), specially in the case of commercially relevant shellfish, marine mammals and other megafauna.

3) Molecular evolutionary and phylogenetic analysis of chromosomal proteins in metazoans. This objective builds on the molecular evolutionary tradition of our group, studying the mechanisms governing the diversification/differentiation of gene and protein families across eukaryotes and their consequences for organismal evolution.

Related research topics are also welcome. Interested students will be required to apply to the Graduate Program in the Dept. of Biological Sciences at FIU (http://biology.fiu.edu/graduate/). Acceptance in the lab will be subject to successful application for Teaching Assistantship funds (TAs). Interested students are also strongly encouraged to apply for graduate research fellowships (e.g., National Science Foundation Graduate Research Fellowship). In order to be considered, qualified candidates must submit a statement of interest, curriculum vitae, unofficial transcripts (GPA scores), GRE scores, TOEFL scores (if applicable) and summary of research interests in a single PDF file to Dr. Eirin-Lopez (jeirinlo@fiu.edu). To receive full consideration, applications and required materials should be received as soon as possible, and never later than November 30th.

 

See ad with more details here.

Personalized epigenetics and the future of health sciences

Posted by | Bioinformatics, Epigenetics, FIU, Omics, Research | No Comments

The latest publication resulting from our ongoing collaboration with the Bioinformatics Research Group (BioRG) at FIU’s School of Computing & Information Sciences is now available!. In this work (Computational methods in epigenetics), BioRG’s postdoc Dr. Vanessa Aguiar-Pulido (L) and Chromevol’s graduate student Victoria Suarez-Ulloa (R) lead a revision describing the main characterisitics of the various types of data generated during epigenetic studies, providing a description of the most common computational approaches used for their integrative analysis. Congratulations for such a wonderful job!

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Asilomar Chromatin, Chromosomes and Epigenetics Conference 2015

Posted by | Chromatin, Conference, Epigenetics, Histones, Research | No Comments

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The 37th edition of the Asilomar Chromatin, Chromosomes and Epigenetics Conference (ACCEC) is rolling and has renewed energies! Dr. Eirin-Lopez has joined the organizing committee of this meeting. Following a long scholar tradition, we’ll be gathering next December in Asilomar, Pacific Grove (CA) to discuss the latest advances in the field. Please check the revamped website for the meeting at:

website: asilomarchromatin.com

facebook: facebook.com/asilomarchromatin

twitter: twitter.com/asilomar_accec

ACCEC is an extraordinary opportunity for graduate students, postdocs and PIs to present their work (oral communications only!) in a relaxed environment in front of a diverse audience. The present edition has special focus on epigenetics and nutrition, among other topics.

Congratulations to Dr. Rivera-Casas!

Posted by | Awards, Chromatin, Evolution, Histones, Marine Biology, Research | No Comments

Yesterday Chromevol’s graduate student Ciro Rivera-Casas defended (with great success!) his Ph.D. dissertation entitled “Histone variants in marine invertebrates: chromatin analysis and characterization of macroH2A and H2A.Z.2 variants in bivalve molluscs”. Ciro joined Chromevol in 2010 and is the second Ph.D. student graduating under Dr. Eirin-Lopez’s supervision. We want to make you all participants of his achievement and we hope he can visit us soon in Miami to tell us more about his research!

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It’s all too much for me to take …

Posted by | Cytogenetics, DNA, Genetics, Marine Biology, Red Tides, Research, Toxicology | No Comments

… when it comes to marine biotoxins!!!

For more than a decade now, we have been studying the effect of pollution on the genome of marine invertebrates, notably the genotoxic effect of marine biotoxins produced during harmful algal blooms. However, while the toxic effect of these compounds is well known, little is known about how much of them is needed and for how long to produce DNA damage. That is precisely what our own Veronica Prego-Faraldo has been studying during the last 2 years.

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In a paper published today in the Journal of Toxicology and Environmental Health, Part A (78:814-824. Jul. 2015. View PDF), she provides in vitro evidence supporting the harmful effect of the biotoxin okadaic acid a low concentrations in specific tissues of bivalve molluscs. This work constitutes the first report investigating the early genotoxic effect of this biotoxin, which soon will be followed by complementary in vivo analyses in the mussel Mytilus.

Environmental epigenetics meets marine invertebrates

Posted by | Chromatin, DNA, Epigenetics, Histones, Marine Biology, Omics, Toxicology | No Comments

Environmental epigenetics investigates the cause-effect relationships between specific environmental factors and the subsequent epigenetic modifications triggering adaptive responses in the cell. Given the dynamic and potentially reversible nature of the different types of epigenetic marks, environmental epigenetics constitutes a promising venue for developing fast and sensible biomonitoring programs. Indeed, several epigenetic biomarkers have been successfully developed and applied in traditional model organisms (e.g., human and mouse). Nevertheless, the lack of epigenetic knowledge in other ecologically and environmentally relevant organisms has hampered the application of these tools in a broader range of ecosystems, most notably in the marine environment.

Fortunately, that scenario is now changing thanks to the growing availability of complete reference genome sequences along with the development of high-throughput DNA sequencing and bioinformatic methods. Altogether, these resources make the epigenetic study of marine organisms (and more specifically marine invertebrates) a reality.

vicky_600x400rodri_600x400We have built on this knowledge to develop a review/perspectives paper on this topic, recently published in the journal Marine Pollution Bulletin. This work (conducted by Victoria Suarez-Ulloa and Rodrigo Gonzalez-Romero, grad student and postdoc at Chromevol, respectively) provides a timely perspective highlighting the extraordinary potential of environmental epigenetic analyses as a promising source of rapid and sensible tools for pollution biomonitoring, using marine invertebrates as sentinel organisms. This strategy represents an innovative, groundbreaking approach, improving the conservation and management of natural resources in the oceans.

 

is it a boy or a girl?

Posted by | DNA, Evolution, Genetics, Molecular biology, Research | No Comments

Sex determination is an extraordinarily complex mechanism. During this process, signals of different nature (genetic, epigenetic, environmental, etc.) interact with each other, often in a hierarchical manner, to produce a male or a female embryo. Historically, the study of sex determination has relied on insect models (specially the fruit fly Drosophila) due to their short generation time as well as to the presence of very well developed genetic toolkits.

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Ten years ago we started a collaboration focused on this topic with the laboratory of Dr. Lucas Sanchez at the CIB-CSIC (Spanish Research Council, Madrid), investigating the evolution of the different components (proteins and genes) of the sex determination cascade in insects. Our latest results on this subject have been published this week by the journal Genetics. In this work we describe an unusual variation in the sex determination mechanism of sciara flies (gnats). More specifically, we find that the gene doublesex (responsible for discriminating between male-specific and female-specific developmental plans) does not seem to play that role in these insects, as suggested by the presence of non sex-specific doublesex transcripts in both males and females. This feature sets Sciara apart from other insects, revealing not only their divergent nature but also the extreme plasticity of sex determination mechanisms in nature.

Chromevol gets seed funds to study oyster responses to red tides

Posted by | DNA, FIU, Oysters, Red Tides, Research, Technology | No Comments

The Biomolecular Sciences Institute at FIU has awarded Chromevol with seed funds to investigate the epigenetic mechanisms participating in the responses of the Eastern oyster (Crassostrea virginica) to Florida Red Tides (caused by the dinoflagellate Karenia brevis) in the Gulf of Mexico. This research is developed in collaboration with Dr. John Berry from the Dept. of Chemistry and Biochemistry, and it complements our work focused on mussels exposed to okadaic acid in the NE Atlantic. Tomorrow we’ll be doing the first oyster exposure experiments in the Ecotoxicology Laboratory (Dr. Gary Rand) at FIU, using K. brevis cultures provided by Dr. Kelly Rein in the core facility for the culture of toxic algae!

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